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L1,microtechnique ,histology

L1,microtechnique, histology 

Lecture video


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MCQ

1-Fixation of fresh tissue is essential to


a-remove water 

b-prevent autolysis 

c-replace alcohol

d-clear the sections

e-keep the cells alive


2-In dehydration of the tissue, we use different grads of


a-formaldehyde 

b-alcohol

c-xylol

d-paraffin wax

e-hematoxylin


3-The aim of dehydration is to 


a-add water to the tissue

b-add paraffin to the tissue

c-remove water from the tissue

d-remove paraffin from the tissue

e-prevent postmortem decomposition


4-Clearing is done by placing the tissues in

 

A-Suza

b- Bouin

c- Zinker

d- Xylol or benzol


5- The most commonly used basic dye is

 

a- Eosin

b- Methylene blue

c- Toluidine blue

d- PAS

e- Hematoxylin

 

6- The most commonly used acidicdye is


a- Eosin

b- Methylene blue

c- Toluidine blue

d- PAS

e- Hematoxylin 



7- Vital stain is

 

a- staining of dead tissue outside the body

b- staining of a living cell inside the body

c- staining of a living cell outside the body

d- staining of a dead tissue inside the body 

(Correct / b)


8- Supravital stain is 


a- staining of a living cell outside the body

b- staining of a living cell inside the body

c- staining of phagocytic cells.


9- Metachromasia


a- staining of a tissue by the color of the original stain

b- staining of a tissue by a color differs from the original stain

c- staining of granules of mast cells by a red color after toludine blue

d- staining of phagocytic cells by trypan blue

e- both b&c


10- Metachromasia is a character of


a- macrophages

b- plasma cells

c- mast cells

d- fibroblasts

e- fat cells


11- Advantages of paraffin sections

 

a- short period of time

b- large pieces of tissues could be used

c- excellent for soft tissues as brain

d- sections are easily stained

e-both a&d 

 

12- Advantages of celloidin sections

 

a- gives perfect sections, as no heat is used 

b- short period of time

c- excellent for soft tissues as brain

d- sections are easily stained 

e- both a&c 


13- Paraffin sections are cut (for light microscope) at a


a- 4-10 A

b- 4-10 nm

c- 4-10 um

d- 4-10 mm

e- 4-10 cm

14- Which of the following is used to make LIPID visible

 

a- Eosin

b- Hematoxylin

c- Sudan III

d- Toluidine blue

e- Mallory stain


15-In paraffin method, clearing is done to:


a- hardens the tissue.

b-prevent putrefaction.

C- preserve the tissue.

d-replace alcohol by paraffin solvent.


16- Concerning the paraffin technique, the most common fixative is:


a-Formaline 

b- Hard paraffin

C-Soft paraffin

d-Xylol


17- The fixative has the following function:


a-Softens the tissue

b- Preserves the structure of the tissue

C- Prevents the staining of the tissue

d-Prevents shrinkage of the tissue


18-One statement is correct


a-Xylol is used for mounting of the sections on the glass slide

b-Dehydration occurs by descending grades of alcohol

C-Xylol is a solvent used for clearing

d-Embedding occurs in the soft paraffin


19- Freezing technique has the following advantage:


a- It takes a short time

b- It gives serial sections

C-It gives thin sections

d-Sections are easy to stain


20- The most common staining system in the histology is

a- Silver stain

b-Orcein stain

C-Hematoxylin and eosin system (H & E)

d-Periodic acid-Schiff reaction (PAS)


21- 10. Which of the following is suitable to examine the fat cells


a-Periodic acid-Schiff reaction (PAS)

b-Ag

C-Sudan Ill

d. Acid phosphatase


22- The aim of dehydration is:

a-Harden the tissue

C-Replace paraffin

b-Clear the tissue

d-Replace H2O with solvent


23-During routine H & E sections, tissue is preserved by:


a- Fixation

b- Dehydration

c-Slicing

d-Staining


Answers 

1-B

2-B

3-C

4-D 

5-E

6-A

7-B

8-A

9-E

10-C

11-E

12-E 

13-C

14-C

15-D

16-A

17-B

18-C

19-A

20-C

21-C

22-D

23-A

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